早园竹叶绿体atpA基因序列及系统进化分析

Phylogenetic Analysis based on Chloroplast atpA Gene Sequences from Phyllostachys propinqua

在构建叶绿体DNA基因组文库的基础上,克隆了早园竹叶绿体atpA基因,与禾本科水稻、玉米等11种植物atpA基因的SNP对比分析发现,早园竹与供试禾本科植物叶绿体atpA基因序列间共有110个SNP位点,其中与水稻Indica,Japonica和野生稻之间仅有1个SNP位点;与玉米、高粱、甘蔗及杂交甘蔗之间有2个SNP位点;而与小麦、大麦、匍茎剪股颖和黑麦草之间的SNP位点较多。基于atpA基因序列和编码氨基酸序列构建的Neighbor-joining系统进化树显示,供试的禾本科植物在系统进化上可划为2个进化类群和4个进化亚群,水稻与早园竹处于同一亚群。表明早园竹在进化关系上与水稻最近,其次与甘蔗、玉米和高粱进化关系较近,而与麦类及其近缘植物的进化关系较远。

Based on the construction of chloroplast genomic library, the atpA gene was cloned from Phyl- lostachys propinqua chloroplast genome, and the atpA gene sequences were compared between P. propinqua and other 11 gramineous plant species collected from GenBank. The results showed that there were 110 single nucleo- tide polymorphisms （SNP） markers between the 12 atpA gene sequences. Among these SNP markers, there was only one SNP marker blasted atpA gene sequence of P. propinqua with atpA gene sequence of Oryza sativa Indica, O. sativa Japonica and O. nivara; there were two SNP markers existed in the sequences of P. propinqua, Zea mays, Sorghum bicolor, Saccharurn officinarum and S. hybrid cuhivar; however, there were more markers between atpA gene sequence of P. propinqua, Triticum aestivum, Hordeum vulgare subsp, vulgate, Agrostis stolonifera and Lolium perenne. The Neighbor-Joining phylogenetic trees based on the atpA gene sequences and coding amino acid sequences indicated that the 12 gramineous plant species could be divided into two major groups and four sub- groups, the P, propinqua and rice were clustered together. It could be concluded from the results that the P. pro- pinqua had the most closest phylogenetic relationship with 0. sativa, the closer relationship was for P. propinqua, Z. mays, S. bicolor, S. officinarum and S. hybrid cuhivar, but the phylogenetic relationship of P. propinqua with wheat and relatives of wheat was far away.