纤维粘连蛋白对膀胱癌细胞生物学行为的影响

Effects of different expression levels of fibronetin on biological behavior of tumor cells

目的研究细胞外基质成分纤维粘连蛋白（ＦＮ）在膀胱肿瘤细胞生长、粘附和侵袭转移中的作用。方法利用转基因技术将ＦＮｃＤＮＡ基因导入低表达ＦＮ的膀胱癌细胞系２５３Ｊ，获得高表达ＦＮ的细胞系２５３ＦＮ，并观察转染前后细胞生长速度的变化。用氮兰四唑盐方法检测细胞与基质的粘附能力；用机械法和细胞分离试验测定细胞的同质粘附性；Ｂｏｙｄｅｎ小室法检测细胞的侵袭能力。结果２５３ＦＮ细胞体外增殖能力减弱；同质粘附能力增强，与基质的粘附能力亦增强。且细胞不易相互分离。体外侵袭实验表明，２５３ＦＮ细胞穿过基底膜的能力减弱。结论随着膀胱癌细胞ＦＮ表达水平的增高，一些恶性表型发生变化，可使其侵袭转移能力受到抑制。

Objective To study the effects of fibronectin(FN) on tumor cell proliferation, adherence and invasion. Methods FN cDNA was introduced into a transitional carcinoma cell line 253J which expressed low level of FN,and 253 FN line which expressed higher level of FN was established. The changes of cellular proliferation in vitro were observed. Adhesiveness to the extracellular matrix was detected by MTT method. Hemotypic adhesion was investigated by detachment assay and invasiveness of the cells was observed with Boyden Chamber before and after FN cDNA gene transfection. Results The growth rate of 253 FN was decreased as compared with its parental cell 253J. Hemotypic adhesion was significantly increased. The adhesiveness of the cells to extracellular matrix was enhanced. Invasion assay showed that invasioness of 253 FN was decreased. Conclusion This study suggested a correlation between FN expression and its biological behavior. With increased FN level, malignant phenotype was changed and invasive ability was inhibited.