陶瓷化骨对人牙囊细胞分化的影响

Ceramic bovine bone affects cellar behavior of purified human dental follicle cells.

目的:培养人牙囊细胞(DFCs),观察陶瓷化骨(CBB)对人牙囊细胞生长和分化的影响。方法:体外分离胚胎期人DFCs,将第三代细胞以CBB为载体立体培养,以平面接种为对照组,用矿化液持续诱导,利用光镜观察细胞生长状态、免疫组织化学观察细胞矿化基质牙骨质附着蛋白(CAP,)及骨涎蛋白抗体(BSP)分泌、Vokassa染色观察矿化情况,了解CBB对DFCs生物学行为的影响。结果:体外连续诱导28d,实验组光镜下可见DFCs与CBB相容性好,材料周边细胞生长密集,并有向BBC趋化迁移现象,附近细胞聚集,形成Vokassa染色阳性的矿化结节,免疫组织化学染色BSP,CAP阳性着色,部分细胞强阳性着色。对照组细胞复层生长,未形成矿化结节,免疫组织化学染色BSP,CAP阴性着色。结论:体外培养条件下,CBB可诱导人DFCs向成骨和成牙骨质细胞分化。

Objective： To investigate the Effect of ceramic bovine bone on behave of purified human dental follicle cells. Method：Human dental follicle cells was cultured in vitro.The subsequent cell passaging were conducted in conditioned medium containing dexamethasone,beta-sodium glycerophosphate and ascorbid acid.with the human dental follicle cells in curture then divided into BBC （in which BBC was added in T-flask）and Hdfc group （without BBC）. Migration was observed under inverted phase microscope and differentiation was observed with Von Kasso stain and. Result： BBC improved the migration and differentiation of cells cultured in vitro, showing no difference in proliferation between two grops. Conclusion： BBC can improved the migration and differentiation of human dental follicle cell.