非同位素PCR-SSCP方法检测横纹肌肉瘤p53基因的敏感性

The Sensitivity and Influence Factor of Non isotopic PCR SSCP in Detecting p53 Gene of Paraffin embedded Rhabdomyosarcomas Tissue

采用非同位素聚合酶链反应—单链构象多态性技术，对３０例横纹肌肉瘤组织ｐ５３基因第５～第８外显了进行检测。结果发现３０例横纹肌内瘤中，７例有异常移动电泳带（２３．３％）。其中２例同时有第７、第８外显子点突变。此法的结果受多种因素的影响，提示要提高阳性率和正确的可重复的结果，必须有严格的实验环境和条件。

Non isotopic polymerase chain reaction (PCR) and single stranded conformation polymorphism (SSCP) method was used to detect point mutation of p53 gene in 30 cases paraffin embedded rhabdomyosarcomas (RMS). The abnormal shifting of the singlestranded DNA (ssDNA) was identified in 7 out of 30 cases.The positive rate was 23.3%,The point mutations were found in exons 7～8 of 2 cases.The results were influenced by many factors as well.It is indicated that strict experimental environment and condition is necessary to improvepositive rate and get right results of repetition.