壳聚糖缓释膜的制备及性能研究

Preparation and properties of chitosan film as a drug sustained-release system

目的探索壳聚糖膜的制备及该膜的缓释、降解性能,并研究该膜对成骨细胞的毒性作用。方法以溶胀度为评价指标,设计正交实验,确定制备壳聚糖膜的最佳参数。检测该膜对牛血清白蛋白的释放率及膜在溶菌酶作用下的降解率。使用噻唑蓝(MTT)法检测该膜对成骨细胞的毒性。结果当稀醋酸浓度为1%、壳聚糖浓度为2mg/mL、三聚磷酸钠浓度为6%、交联时间为1h时,膜的溶胀度最小。该膜8d时释放率达33.13%,4周内可降解36.73%。该膜对胎鼠成骨细胞的毒性作用为0级或1级。结论用最佳参数制备的膜具有良好的缓释能力、可降解性,对胎鼠成骨细胞无毒性。

Objective To develop a best chitosan film for using as a drug sustained-release system through the evaluation of the sustained-release property, degradation property, and cytotoxicity to osteoblast. Methods Orthogonal experiments were designed to determine the best combination of chitosan film preparations. Drug release rate was determined with Coomassie brilliant blue G250. In a separate study, chitosan films were placed into the test tubes with buffer solution and 107 U/L lysozyme. The degradation rate was calculated. Osteoblasts derived from fetal rat ealvarial were cultured on chitosan films. Cell proliferation was tested by methyl thiazolyl tetrazolium （MTT） assay. The relative growth rate was calculated and the cytotoxicity was graded. Results The best processing condition was 1% acetic acid, chitosan concentration of 2 mg/mL, 6% sodium tripolyphosphate （STPP） concentration, and crosslinking time of one hour. The resulting chitosan film released 33.13% of bovine serum albumin （BSA） within 8 d, 36.73% of BSA within four weeks and the eytotoxicity grade was 0 or 1. Conclusion This chitosan film possesses good sustained release property, and a good degradation rate.