辛伐他汀对人牙周膜细胞成骨活性的影响

Effects of Simvastatin on osteoblast activity of human periodontal ligament cells

目的研究辛伐他汀对人牙周膜细胞成骨活性的影响。方法将第3代人牙周膜细胞在条件矿化培养液中诱导培养,同时加入不同浓度的辛伐他汀,将其分为A组(0mol/L)、B组(1×10-9mol/L)、C组(1×10-8mol/L)、D组(1×10-7mol/L)、E组(1×10-6mol/L),分别检测碱性磷酸酶活性、骨桥蛋白(OPN)表达,并对矿化结节进行计数。结果辛伐他汀各浓度组(1×10-9、1×10-8、1×10-7、1×10-6mol/L)均能促进人牙周膜细胞的成骨分化和矿化,其中1×10-8、1×10-7、1×10-6mol/L与对照组比较,差异有统计学意义(P<0.05),1×10-7mol/L的辛伐他汀组促进成骨活性的作用最明显。结论适宜浓度的辛伐他汀可以有效促进人牙周膜细胞的成骨活性。

Objective To study the effects of Simvastatin on the osteoblast activity of human periodontal ligament （PDL） cells. Methods The third passage human PDL were cultured in conditional mineralization medium with different concentrations of Simvastatin. The cells were divided into A group（0mol/L）, B group（1×10^-9mol/L）, C group （1×10^-8mol/L）, D group（1×10^-7mol/L） and E group（1×10^-6mol/L）. Alkaline phosphatase（ALP） activity, osteopontin （OPN） and capability of mineralization were measured. Results Differentiation osteoblast and mineralization of human PDL were improved in all treatment groups with different concentrations of Simvastatin（1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6mol/L）. Compared with control group, statistically significant differences were found in 1×10^-8mol/L, 1×10^-7mol/L and 1×10^-6 mol/L groups（P〈0.05）. The maximum effect was observed at the concentration of 1×10^-7 mol/L. Conclusion Optimal concentration of Simvastatin can improve the osteoblastic activity of human PDL.