口腔癌细胞诱导的淋巴管内皮细胞基因表达谱的变化

Altered gene expression of lymphatic endothelial cells in oral cancer

目的探讨在口腔癌细胞影响下淋巴管内皮细胞分子表型的变化。方法利用体外共培养模型,将淋巴管内皮细胞与口腔癌细胞进行共培养,模拟肿瘤中淋巴管内皮细胞的变化。应用Affymetrix U133Plus2.0基因芯片对肿瘤组织中淋巴管内皮细胞(TLEC)与淋巴管内皮细胞(LEC)基因表达的差异进行了检测和比较。并对功能相似的表达差异基因进行分类。结果差异基因表达谱共发现了677个基因表达差异在1倍以上,其中在TLEC中表达上调的基因有384条,下调的基因有293条。这些基因与细胞黏附、凋亡、运动、发育及血管生成有关。同时这些基因还参与细胞的信号传导、免疫应答、细胞代谢等过程。结论LEC与TLEC在分子水平是有差别的。以此为基础,可以针对淋巴管内皮细胞进行靶向阻断,达到治疗口腔癌淋巴道转移的目的。

Objective To explore altered molecular phenotype of lymphatic endothelial cells （LEC） induced by oral cancer cells. It is relevant to develop therapeutic strategies for blocking oral cancer spread through lymphatic vessels. Methods LEC were co-cultured with oral cancer cells in a model. Differential gene expression profiles between tumor-derived lymphatic endothelial cell （TLEC） and LEC were analyzed by Human Genome U133 Plus 2.0 GeneChip arrays. Differential expression genes of functional similarity were classified. Results Differential gene expression profiles revealed that 677 unique genes had at least a twofold change in expression level between the two groups, 384 were overexpressed, and 293 were underexpressed in TLEC. These genes were related to cell adhesion, apoptosis, cell motility, development, and angiogenesis. In addition, some genes were involved in signal transduetion, immune response, cell metabolism, and so on. Conclusion LEC and TLEC are distinct at the molecular level, A novel therapeutic strategy of lymphatic metastasis is encouraging and anticipated based upon manipulation of LEC responses to oral cancer cells.