碱性成纤维细胞生长因子对牙周膜细胞表皮生长因子受体基因表达的影响

Effect of basic fibroblast growth factor on the gene expression of epidermal growth factor receptor by periodontal ligament cells in culture

目的研究碱性成纤维细胞生长因子(bFGF)对人牙周膜细胞(PDLC)表达表皮生长因子受体(EGFR)的影响,探讨bFGF在牙周组织分化再生中的意义。方法体外原代培养人PDLC,有限稀释法形成单细胞克隆,用外源性bFGF刺激单细胞克隆,采用逆转录聚合酶链反应(RT-PCR)检测克隆细胞内EGFR基因表达的变化。结果bFGF促进人PDLC内EGFR mRNA的合成,并且随着质量浓度的增加促进作用增强。结论bFGF对EGFR的促进作用很可能是牙周炎损伤修复过程中一个重要的调节因素,为牙周组织分化再生提供部分理论基础。

Objective To study the effect of basic fibroblast growth factor （bFGF） on the gene expression of epidermal growth factor receptor （EGFR） by periodontal ligament cells （PDLC） in culture and discuss the effect of bFGF in cell differentiation and periodontal regeneration. Methods Human PDLC were cultured in vitro and cell clone was obtained by the method of limiting dilution. PDLC were stimulated by bFGF, and then gene expression of EGFR was assessed by reverse transcription-polymerase chain reaction （RT-PCR）. Results The mRNA expression of EGFR was promoted by bFGF and the effect was dose-dependent. Conclusion The promotion effect of the mRNA expression of EGFR in PDLC may be one of important factors which participate in the healing process of periodontitis and provide partly theoretical basis of cell differentiation and periodontal regeneration.