疏水蛋白HFBI及胶原共修饰的PLGA对神经干细胞贴附和生长的影响

The Effect of PLGA Modified by Hydrophobin HFBI and Collagen TypeⅠ on the Adhesion and Growth of Neural Stem Cells in Vitro

目的:探讨神经干细胞(NSCs)在经疏水蛋白HFBI和Ⅰ型胶原联合修饰的聚乳酸-乙醇酸(PLGA)膜上贴附和生长的可行性。方法:将神经干细胞分离、纯化及扩增后,在无血清和有血清的条件下,分别接种到PLGA膜和经疏水蛋白HFBI和Ⅰ型胶原联合修饰的PLGA膜上,观察神经干细胞的贴附、生长情况。结果:经过培养,无血清组中,在PLGA膜上没有观察到神经干细胞;在疏水蛋白HFBI与胶原联合修饰的PLGA膜上,观察到有少量的神经干细胞呈圆形状贴附,生长。血清组中,在PLGA膜以及疏水蛋白HFBI和胶原联合修饰的PLGA膜上,均观察到存在大量细胞生长,并且在疏水蛋白HFBI和胶原联合修饰的PLGA膜上,观察到更多含有突起的细胞。结论:疏水蛋白HFBI和Ⅰ型胶原联合修饰PLGA膜有利于促进神经干细胞贴壁,在血清的条件下明显促进神经干细胞生长和分化。

Objective： To investigate the adhesion and growth of neural stem cells （NSCs） on the native PLGA which was modified by hydrophobin HFBI and collagen type Ⅰ. Methods： NSCs were cultured on the native PLGA, which was modified by hydrophobin HFBI and collagen type Ⅰ, with or without serum, respectively. The adhesion and growth of NSCs were observed. Results： In the medium without serum, no NSCs adhered to the surface of PLGA and only a few cells adhered to and grew on the surface of PLGA modified by hydrophobin HFBI and collagen. In the medium supplemented with serum, most cells adhered to and grew on the surface of both PLGA and PLGA modified by hydrophobin HFBI and collagen. Besides, more cells with cellular processes were observed on the PLGA substrate modified by hydrophobin HFBI and Collagen. Conclusion： The PLGA modified by hydrophobin HFBI and collagen typeⅠ modifying PLGA was demonstrated to promote the adhesion and growth of NSCs. The NSCs could differentiate at the presence of serum.