心肌内双层多孔生物可降解性药物缓释支架的制备(英文)

Preparation of intramyocardial bilayered porous biodegradable drug delivery stents

目的:制备心肌内双层多孔生物可降解性药物缓释支架,评估其对透室壁性心肌血管重建术后心肌孔道的作用效果。方法:以聚己内酯为材料,以牛血清白蛋白为模型药物,以聚乳酸-聚乙醇酸共聚物为药物载体,制备成生物可降解性药物缓释支架。采用考马斯亮蓝试剂法对支架上牛血清白蛋白含量及体外释放量进行测定,万能材料测定仪测定支架的力学性能。制备猪慢性心肌局部缺血模型,体内评估该支架在透室壁性心肌血管重建术后对心肌孔道的作用效果。结果:该支架牛血清白蛋白携带量为每支架10mg,30d后牛血清白蛋白释放量达80%,支架压缩80%时承受的应力为1.2MPa,在透室壁性心肌血管重建后可保持心肌孔道通畅。结论:成功制备心肌内双层多孔生物可降解性药物缓释支架,能承受心肌压力并达到缓慢控制释放药物的效果,可维持透室壁性心肌血管重建后的心肌孔道通畅。

OBJECTIVE： To prepare an intramyocardial bilayered porous biodegradable drug delivery stent and to evaluate its effects on myocardial channel after transmyocardial revascularization （TMR）. METHODS： A biodegradable drug delivery stent was prepared by using poly （^-caprolactone） （PCL）, bovine serum albumin （BSA） and pely （D, L-lactide-co-glycolide） （PLGA）. The levels of BSA in stent and released in vitro were determined by the Coomassie brilliant blue assay. The mechanical strength of stent was tested by universal material testing machines. Porcine models of chronic myocardial ischemia were created to evaluate the effects of this stent on myocardial channel after TMR. RESULTS： Each bilayered porous stent could carry 10 mg BSA and release about 80% of BSA after 30 days, The stent diminished 80% of initial scale under the stress of 1.2 MPa. It could keep myocardial channel patency after TMR. CONCLUSION： An intrarnyocardial bilayered porous biodegradable drug delivery stent was successfully prepared. It could sustain the pressure from the heart and maintain myocardial channel patency after TMR.