大鼠肌源性干细胞移植对失神经腓肠肌萎缩进程的影响

Effects of muscle-derived stem cell transplantation on denervated gastrocnemius atrophy in rats

背景:目前,国内外有关肌源性干细胞移植的研究主要集中在治疗肌营养不良性萎缩症方面,尚未见涉及将肌源性干细胞应用于失神经肌萎缩综合治疗的报道。目的:探讨大鼠肌源性干细胞移植对失神经腓肠肌萎缩进程的影响。设计、时间及地点:随机对照动物观察,于2007-02/2008-07在沈阳医学院完成。材料:成年健康雄性Wister大鼠19只,由沈阳医学院实验动物中心提供。方法:取3只大鼠,无菌条件下切取肱三头肌,体外分离培养肌源性干细胞,贴壁法纯化扩增。余16只大鼠均切断右后肢胫神经,建立失神经腓肠肌模型,分为2组:细胞移植组于已切断的胫神经支配的腓肠肌的内外侧注射经DAPI标记的肌源性干细胞悬液0.2mL,模型对照组于相同位置注射等量生理盐水,培养4周。主要观察指标:采用计算机生物信号采集处理系统测定反映肌张力的腓肠肌阈强度恢复率和最适强度恢复率变化,通过电子天平计算肌湿质量残存率,应用图像分析系统测定肌纤维横截面积残存率。结果:与模型对照组比较,细胞移植组失神经腓肠肌的阈强度恢复率、最适强度恢复率均明显降低(P<0.01,P<0.05),肌湿质量残存率、肌纤维横截面积残存率均明显升高(P<0.01)。结论:肌源性干细胞移植对大鼠失神经肌萎缩进程有较明显的延缓作用。

BACKGROUND： Studies concerning muscle-derived stem cell transplantation mainly focused on treatment of muscular dystrophy. There are no studies on muscle-derived stem cells for treatment of denervated amyotrophy combined therapy. OBJECTIVE： To explore effects of muscle-derived stem cell transplantation on denervated gastrecnemius atrophy. DESIGN, TIME AND SETTING： The randomized controlled animal study was performed at the Shenyang Medical College from February 2007 to July 2008. MATERIALS： A total of 19 adult healthy male Wistar rats were supplied by Animal Experimental Center, Shenyang Medical College. METHODS： Triceps brachii was sterilely collected from 3 rats, and muscle-derived stem cells were isolated in vitro, and purified by adherence method. The tibial nerve of right posterior limb was cut in remaining 16 rats to establish animal models of denervated gastrocnemius, in the cell transplantation group, 0.2 mL DAPl-labeled muscle-derived stem cell suspension was infused into the denervated gastrocnemius. The same volume of saline was injected into the model control group, for 4 weeks. MAIN OUTCOME MEASURES： Computer biological signal collection processing system was used to measure recovery rate of threshold intensity of gastrocnemius and the optimal intensity recovery rate. Electronic balance was utilized to calculate residual rate of wet weight. Image analysis system was used to measure residual rate of cross section area. RESULTS： Compared with the model control group, the recovery rate of threshold intensity of denervated gastrocnemius and the optimal intensity recovery rate were significantly lower in the cell transplantation group （P 〈 0.01, P 〈 0.05）. Residual rate of wet weight and residual rate of cross section area were significantly higher （P 〈 0.01）. CONCLUSION： Muscle-derived stern cell transplantation can delay atrophy process of denervated skeletal muscle in rats.