食管脱落细胞DNA分析对食管癌诊断价值的探讨

Value of DNA analysis of exfoliated esophageal cells in diagnosis of esophageal carcinoma

目的:探讨通过流式细胞术进行食管脱落细胞DNA分析,在食管癌诊断中的价值。方法:用食管拉网法获得61例食管癌和31例非食管癌患者食管脱落细胞,记录和计算DNA指数(DI)、G2/G1、S期比率(SPF)和细胞增殖活性指数(PI)等,同时结合肿瘤的临床病理学特征,并与脱落细胞常规细胞学检查进行对比。结果:食管癌组DI为1.31±0.23,对照组为1.00±0.03;异倍体(An)出现率为70.7%,高于对照组4.8%;食管癌组G2/G1为2.071±0.153,对照组为1.996±0.099;并且SPF和PI两组指标的差异均有统计学意义,P<0.05。结论:食管脱落细胞DNA增殖活性指标对食管癌普查及提高早期诊断率具有一定的应用价值。

OBJECTIVE： To evaluate the clinical significance of DNA analysis of extoliated esophageal cells in early diagnosis of esophageal carcinoma. METHODS： The exfoliated cells were obtained by the esophagus fleece-pulling. The single-cell suspension was made by mechanical decentralization and then detected by the flow cytometry for DNA analysis. DNA index （DI）, G2/G1, S-phase fraction （SPF）, proliferation index （PI）, etc were recorded and calculated. The clinical evaluation was made by comparing with the routine cytology examination. RESULTS： The DI of esophageal carcinoma group and the control group was 1.31±0.23 and 1.00±0.03. The Aneuploid rate of esophageal carcinoma group was 70. 7%, while that of control group was 4.8%. The G2/G1 for esophageal carcinoma group was 2. 071 ± 0. 153 and that for control group was 1. 996±0. 099. There was significant difference between two groups （P 〈 0. 05） in SPF and PI. CONCLUSION： DNA analysis of exfoliated esophageal cells can raise the early diagnostic rate of esophageal carcinoma.