低剂量X射线照射诱导G_1期阻滞的适应性反应

Adaptive response of G 1 arrest induced by low dose X rays.

目的观察低剂量Ｘ射线照射能否诱导Ｇ１期细胞阻滞。方法采用碘化丙啶（ＰＩ）荧光探针标记细胞，流式细胞术（ＦＣＭ）检测并分析细胞周期。结果证实１．５ＧｙＸ射线全身照射后１２小时，小鼠胸腺细胞发生明显Ｇ１期阻滞。而０．０７５Ｇｙ低剂量预照射可明显减轻其后１．５Ｇｙ攻击剂量所致Ｇ１期阻滞。离体照射ＥＬ－４淋巴瘤细胞也得到类似结果。结论０．０７５ＧｙＸ射线照射能诱导胸腺淋巴细胞及ＥＬ－４细胞Ｇ１期阻滞的适应性反应。

bjective\ To investigate whether or not adaptive response of G 1 arrest could be induced by low dose radiation.\ Methods\ Flow cytometry (FCM) was used to analyze cell cycle stained with propidium iodide (PI).\ Results\ \ It was found that 12h after 1.5 Gy whole body X irradiation,G 1 arrest of thymocytes took place in Kunming mice.However,G 1 arrest was diminished when a 0.075 Gy conditioning dose was given 6h before 1.5 Gy challenge dose.The same result was also revealed in EL 4 lymphoma cell line.Conclusion\ Adaptive response of G 1 arrest could be induced by low dose X rays.