从IL-2和Bcl-2基因转录水平探讨模拟失重鼠T淋巴细胞功能改变的机制

Study on Mechanisms of T Lymphocyte Function Changes in Mice under Simulated Weightlessness in Terms of IL-2 and Bcl-2 Gene Transcription.

为探讨模拟失重条件下Ｔ淋巴细胞功能改变的机制，观察了小鼠脾脏Ｔ淋巴细胞增殖能力（ＭＴＴ比色法）、ＩＬ－２产生（生物学测定法）、ＩＬ－２基因（点杂交）和Ｂｃｌ－２癌基因（逆转录ＰＣＲ）转录的变化。结果显示：（１）模拟失重７、１４ｄ小鼠脾脏Ｔ淋巴细胞增殖能力和ＩＬ－２的产生降低，１４ｄ时有显著性（Ｐ＜０．０１）。表明模拟失重降低Ｔ淋巴细胞功能。（２）模拟失重７、１４ｄ小鼠脾脏Ｔ淋巴细胞ＩＬ－２和Ｂｃｌ－２基因转录水平降低，１４ｄ时有显著性（分别为Ｐ＜０．０５和Ｐ＜０．０１）。表明模拟失重通过抑制ＩＬ－２基因转录抑制ＩＬ－２的产生，ＩＬ－２和Ｂｃｌ－２基因对模拟失重引起的Ｔ淋巴细胞功能的降低起一定的作用。

To understand the mechanisms of T lymphocyte function changes under simulated weightlessness, T lymphocyte proliferation(MTT assay),IL-2 production(biological assay),IL-2 gene(dot blot)and Bcl-2 oncogene (RT-PCR) transcription of splenic cell were observed in mice.The results showed that on the 7 th and 14 th day of simulated weightlessness T lymphocyte proliferation and IL-2 production decreased and significant on the 14 th day;on the 7 th and 14 th day of simulated weightlessness IL-2 and Bcl-2 gene transcription decreased,significant on the 14 day. It demonstrated that simulated weightlessness inhibits IL-2 production by decreasing IL-2 gene transcrition.IL-2 and Bcl-2 gene may be regulators of T lymphocyte function under simulated weightlessness.