前S_2抗原、HBV血清标志物与HBV-DNA检测结果的相关性探讨

Correlation of detecting results of HBV-DNA and Pre-S_2 protein in diagnosis of hepatitis B virus infection

目的研究前S2抗原、HBV血清标志物检出情况与HBV-DNA结果之间的关系。方法分别进行前S2抗原、HBV血清标志物5项的检测,同时对乙肝患者运用PCR检测HBV-DNA的含量。结果大三阳组前S2抗原(+)检出率和HBV-DNA阳性检出率均高于小三阳组,且与小三阳组比较差异均有统计学意义;前S2抗原(-)组血清HBV-DNA检出率为89%;前S2抗原(+)组与血清HBV-DNA差异有统计学意义(P<0.010)。结论在前S2抗原、HBV血清标志物与HBV-DNA检测中应联合应用两种方法进行检测,提高检出率,为判断乙肝病毒的复制、传染性以及指导临床治疗提供更可靠的实验依据。

Objective To investigate the relationship between detection of HBV-DNA and routine detection of HBV. Methods HBV was routinely determined with ELISA in serum samples. HBV DNA was detected with PCR from cases of hepatitis B. Results The parameters of HBsAg（ ＋ ）, HBeAg（ ＋ ） and HBcAb（＋） were significantly different from HBsAg（ ＋ ）, HBeAb（ ＋ ） and HBcAb（ ＋ ）. The detecting rate of HBV-DNA was 89 % in the patients of preS2 （ - ）. Furthermore, there were statistical difference between preS2 （＋） and HBV-DNA. Conclusion The two methods should be simultaneously used in the detection of HBV infection for increasing the detecting rate and providing more reliable evidence to judge HBV infection, duplication,infectivity and its treatment in clinical practice.