实验分离、鉴定人感染猪链球菌2型的临床意义

Clinical significance of experimental isolation and identification in human streptococcus suis 2 infections

目的探讨实验分离、鉴定人感染猪链球菌2型(S.Suis2)的临床价值。方法所有标本均先直接涂片革兰染色镜检查找细菌;然后根据细菌的菌落形态、生化反应、药敏结果、血清学及PCR试验等进行诊断鉴定。结果22例疑似S.Suis2菌感染患者共采集标本30份(血液22份,脑脊液8份),血液培养出S.Suis2菌15株(68.2%),脑脊液培养出S.Suis2菌5株(22.7%),血液、脑脊液同时培养出S.Suis2菌3株(13.6%)。结论实验分离、鉴定S.Suis2菌有利于感染患者的诊断和治疗。

Objective To study the clinical value of experimental isolation and identification in human streptococcus suis 2 （S. suis 2） infections. Methods For one thing, the bacterium in all specimens was found directly by Garm＇s stained film microscopy. For another, the bacterium was identified according to the results of colonial morphology,biochemical reactions,drug susceptibility, serological as well as PCR determination. Results Thirty specimens were collected from twenty-two suspicious patients with human streptococcus suis 2 infections（22 blood, 8 cerebrospinal fluid）. The prevalences were 68.2 % （15/22） for blood, 22.7% （5/22） for cerebrospinal fluid and 13.6 % （3/22） for both blood and cerebrospinal fluid,respectively. Conclusion There are a lot of benefits about diagnosis and therapy of human streptococcus suis 2 infections by experimental isolation and identification.