Dual effects of pentobarbital on rat sacral dorsal commissural neurons in vitro

Dual effects of pentobarbital on rat sacral dorsal commissural neurons in vitro 1

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AIM: To study the effects of pentobarbital (PB) on acutely dissociated rat sacral dorsal commissural neurons (SDCN). METHODS: Nystatin perforated patch clamp recording was used. RESULTS: (1) At a holding potential of -40 mV, PB induced inward Cl - current ( I PB ) in a concentration dependent manner with a EC 50 (95 % confidence limits) of 416 (385-477) μmol·L -1 and a Hill coefficient of 1 08. (2) Picrotoxin reversibly blocked I PB . (3) The reversal potential of I PB was close to the Cl - equilibrium potential. (4) PB enhanced GABA induced Cl - influx ( I GABA ). In the presence of PB 30 μmol·L -1 , the EC 50 (95 % confidence limits) of I GABA decreased from 6 9 (5 4-8 4) μmol ·L -1 to 3 5 (2 9-4 1) μmol·L -1 . CONCLUSION: PB had dual effects on SDCN, facilitated GABA A receptor mediated currents, and at higher concentrations induced Cl - influx itself.