细胞质雄性不育海岛棉恢复系恢复基因的遗传分析

Inheritance of Fertility Restoration for Cytoplasmic Male Sterility in a New Gossypium barbadense Restorer

【目的】在陆地棉细胞质雄性不育系与海岛棉种质材料进行测恢筛选时,选得一个对不育有恢复力的海岛棉材料"海R",本研究的目的是了解其育性恢复的遗传方式。【方法】构建23种不同的F2群体和回交群体,分析和明确恢复基因的遗传。【结果】"海R"恢复基因属孢子体遗传,由一个主效显性基因(RfB)控制。在恢复基因呈隐性时不育胞质不但对雄配子的传递有影响,而且对雌配子的传递也有影响。【结论】推测此海岛棉恢复系的育性恢复基因来源于哈克尼西棉基因组,在陆地棉与海岛棉的种间杂种优势利用中具有应用价值。

[Objective] In order to clarify inheritance mechanism of fertility restoration for cytoplasmic male sterility （CMS） in a new Gossypium barbadense restorer line‘Hai R＇which was found in the fertility test crossing of G hirsutum CMS lines with G barbadense germplasms. [Method] Twenty three fertility segregation populations of F2 and backcrossing were used to analyze the inheritance of fertility restoring gene（s） of ‘Hai R＇. [Result] The result showed that ‘Hai R＇ had one major dominant gene （RfB） to control the CMS fertility restoration and this fertility restoration gene functioned at the sporophytic level. The sterile cytoplasm background might not only influence the transmission rate of male gamete but also that of female gamete when the restorer gene was recessive. [Conclusion] It could be deduced that this fertility restoration gene might come from G. harknessii cotton,‘Hai R＇ is of value in the application of cotton interspecific hybrid breeding.