摘要
[目的]建立以高效液相色谱法测定小儿消退糖浆中的大黄酚含量的方法。[方法]色谱为C18色谱柱(4 mm×250 mm,5μm),流动相为甲醇—水—磷酸—戊烷磺酸钠(80∶20∶0.1∶0.1),流速1.0 mL/min,检测波长为254 nm,柱温为室温。[结果]大黄酚检测浓度在5～25 mg/L范围内与峰面积值线性关系良好(r=0.999 9),平均回收率为100.13%(RSD=1.1%)。[结论]本方法可用于消食退热糖浆的质量检测。
[Objective] To establish HPLC method for determination of chrysophanol in pedo-digestion ＆ fever-bringing-down syrup. [Method] The determination was performed on Cls column, the mobile phase consisted of methanol-water-phosphoric acid-sodimn pentanesulfonic acid （80：20：0.1：0. l g） with flow rate at 1.0 mL/min and detection wavelength at 254 nm under room tempterature. [Results] Chrysophanol had good linear relation in the range of 5-25 mg/L （r=0.999 9）, the average recovery was 100.13%（RSD=I.I%）. [Conclusion] The method can be used to control the quality of digestion ＆ bringing down a fever syrup.
出处
《天津中医药大学学报》
CAS
2009年第2期89-91,共3页
Journal of Tianjin University of Traditional Chinese Medicine
关键词
高效液相色谱法
小儿消退糖浆
大黄酚
含量
HPLC
Digestion ＆ bringing down a fever syrup
Chrysophanol determination
