胃腺癌中人硒结合蛋白-1 mRNA表达水平的变化及意义

Expression and clinical significance of SBP1 mRNA in gastric carcinoma

目的初步探讨人硒结合蛋白1(SBP1)mRNA在胃腺癌组织和癌旁正常组织中的表达及其与临床病理因素的关系,评价SBP1在胃癌发生、发展中的作用及意义。方法采用RT-PCR方法检测SBP1 mRNA在16例胃腺癌手术患者的癌组织、相应癌旁正常组织中的表达水平,分析其表达与临床病理因素之间的关系。结果RT-PCR结果显示,癌旁正常组织中SBP1 mRNA的表达明显强于对应癌组织。经检测的16例胃腺癌组织中SBP1 mRNA表达水平为0.5367±0.0930,而相应癌旁正常组织为0.8429±0.0568,两组差异具有显著性(P=0.005)。SBP1 mRNA的低表达与胃腺癌的分化程度、临床分期、患者的年龄、性别、TNM分期等均无关。结论SBP1 mRNA在胃腺癌中表达明显减少甚至缺失,这种变化可能在胃癌的发生中起到了关键作用。

Objective To investigate the expression of human selenium binding protein 1 （SBP1） mRNA in gastric carcinoma and its clinicopathological significance in the differentiation and metastasis of gastric carcinoma. Methods The expression of SBP1 mRNA was determined by reverse transcriptase-polymerase chain reaction （RT-PCR） in 16 patients with gastric carcinoma and adjacent non-cancerous samples as controls. Correlation of SBP1 mRNA expression and clinicopathologic variables was analyzed. Results RT-PCR demonstrated that the expression levels of SBP1 mRNA in gastric carcinoma samples were significantly lower than those in adjacent non-cancerous tissues. SBP1 mRNA/β-actin mRNA were 0. 5367 ±0. 0930 and 0. 8429±0. 0568 respectively, the differences among different diagnostic groups were statistically significant （P=0.005）. The down-regulated expression of SBP1 mRNA had no correlation with differentiation of gastric carcinoma, metastasis, age, sex, clinical stage and TNM stage. Conclusion Greatly reduced SBP1 expression levels were observed in gastric cancer tissues. The loss of SBP1 may be an early event in tumorgenesis.