糖尿病胃轻瘫大鼠胃窦内ICC和SP变化的实验研究

Research on the changes of interstitial cells of Cajal and substance P in gastric antrum of diabetic gastroparesis rats

目的观察Cajal间质细胞和P物质在糖尿病胃轻瘫大鼠胃窦的改变,进而探讨糖尿病胃轻瘫发生的相关机制。方法SD大鼠30只,随机分为正常对照组(n=10)和糖尿病组(n=20)。用链脲佐菌素建立大鼠糖尿病胃轻瘫模型,3个月后用放射性核素(99mTc)灌胃测定大鼠胃液体排空率,取正常对照组及模型组大鼠胃窦标本,应用免疫组化染色法检测c-kit阳性Cajal间质细胞和P物质在大鼠胃窦的变化,应用彩色病理图像分析软件进行分析,并行统计学比较。结果与正常大鼠相比,糖尿病大鼠胃排空明显延迟(P<0.01),胃窦c-kit阳性Cajal间质细胞减少(P<0.01),P物质表达减少(P<0.01)。结论糖尿病胃轻瘫与Cajal间质细胞和P物质的变化有一定的相关性。

Objective To investigate the changes of interstitial cells of Cajal （ICC） and substance P （SP） in the myenteric plexus of gastric antrum in the rat diabetic gastroparesis model and to explore their possible role in the pathogenesis of diabetic gastroparesis. Methods Thirty SD rats were randomly divided into the normal control group （n = 10） and the diabetic model group （n = 20）. Rats in the diabetic gastroparesis model group were intraperitoneal injected with streptozotocin （STZ） for 3 months. The liguid food emptying rate was assessed by gastrogavage gaving radioactive ^99mTc. The tissue specimens from both the control group and the diabetic model group were analyzed by immunohistochemical assay for c-kit positive ICC and SP. Quantitative image analysis software was used for statistical analysis. Results The gastric emptying in diabetic gastroparesis group decreased significantly （P 〈 0.01 ） compared with normal group. C-kit positive ICC were reduced （P 〈 0.01 ） and the expression of SP was decreased （P 〈 0.01 ） in diabetic gastroparesis group compared with normal group. Conclusion The diabetic gastroparesis is related with the decrease of interstitial cells of Cajal and substance P in gastric antrum.