端粒酶与Ki-67及p53在大肠癌和大肠腺瘤病变中的关系

Correlation among telomerase, Ki-67 and p53 in colon carcinoma and adenoma

目的:探讨端粒酶与Ki-67、p53在大肠癌和腺瘤的表达及发生、发展机理。方法:用组织芯片原位杂交的方法在分子学水平对细胞的端粒酶进行检测,同时用免疫组织化学法对Ki-67,p53进行检测。结果:72例大肠癌端粒酶表达的阳性率为77.78%,Ki-67阳性率88.89%,p53阳性率63.89%;36例大肠腺瘤端粒酶表达阳性率8.3%,p53阳性率22.22%,Ki-67阳性率13.89%。端粒酶和Ki-67、p53的阳性表达在大肠癌和大肠腺瘤之间的差异有显著性(P<0.05)。结论:端粒酶和K-i67、P53在大肠细胞癌变过程中有一定的协同作用。

Objective：To analyse the expression of tetomerase,Ki-67 and p53 in malignant and benign colon lesions by investigating the effect of the telomerase during the development of colon carcinoma. Methods ：The expression of telomerase was examined by using in situ hybridization （ISH）. The immunohistochemistry method was used to detect Ki-67 and p53. Results：Seventy-two cases of colon carcinoma were analysed. The positive ratio of telomerase was 77.78%. The positive ratio of Ki-67 was 88.89% ,while p53 was 63.89%. Thirty-six cases of colon adenoma expressed the telomerase, Ki-67 and p53. The positive ratio of telomerase was 8.3%. The positive ratio of Ki-67 was 13.89%,while p53 was 22.22%. The difference of the expression of telomerase,Ki-67 and p53 in colon between carcinoma and adenoma had statistic significant （P〈0.05）.Conclusion ：There is the correlation among telomerase, Ki-67 and P53 during the beginning and development of carcinoma in colon.