激活态雪旺细胞体外培养与纯化的新方法

A NEW WAY OF CULTURING AND PURIFYING SCHWANN CELL IN VITRO

目的:探求一种快速培养高纯度激活态雪旺细胞的新方法。方法:w istar大鼠坐骨神经体内切断后7d,取预变性坐骨神经,综合运用混合酶消化、三次组织块移植法,原代提取雪旺细胞,低酶双差速纯化雪旺细胞,不做任何处理的坐骨神经作为对照组。结果:此法培养的雪旺细胞纯度为97.8%左右,细胞增殖能力是未做预变性组的5倍。结论:体内预变性、体外两步酶消化-三次组织块移植结合低酶双差速纯化是一种简便、快速的原代培养、纯化雪旺细胞的方法。

Objective：To explore a new way of culturing and purifying schwann cell in short time. Methods：the sciatic nerves of wistar rats were cutting off in vivo,7 days later, to take pre - degeneration sciatic nerves, useing of two - step enzymatic digestion - three times tissue transplantation to extracle primary Schwann ceils, then purified Schwann cells with low- concentration, the sciatic nerves without treating as a control group. Results：The purity is about 97.8% to culture Schwann cells with this method, Schwann cell grew faster from the treated nerve than the nerve without treating. Conclusion： It is a simple, fast way to obtain and purify the Schwanncell by the pre - degeneration vivo and two - step enzymatic digestion in vitro, combination of tissue transplantation and low - concentration enzyme purification.