平肝解毒散对急性肝损伤保护作用的研究

THE EFFECTIVENESS OF PING GAN JIE DU SAN TO PROTECT ACUTE LIVER INJURY

目的:观察平肝解毒散(其主要成分柴胡)对急性肝损伤的保护作用,为临床应用提供科学依据。方法:四氯化碳(CCL4)和硫代乙酰胺(TAA)小鼠腹腔注射造成急性肝损伤实验模型,检测血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST),并做肝脏病理切片分析。结果:CCL4模型组:平肝解毒散中、大剂量组(4g/kg、8g/kg)小白鼠的丙氨酸氨基转移酶(ALT)与模型组比较有显著差异,呈剂量依赖关系,大剂量效果最好;肝的病理切片与模型组比较肝细胞坏死程度减轻,肝细胞变性减轻。TAA模型组:平肝解毒散小剂量组小白鼠的丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)与模型组比较有显著差异,小剂量有效;肝的病理切片与模型组比较肝细胞坏死程度减轻,肝细胞变性减轻。结论:平肝解毒散对CCL4和TAA引起的急性肝损伤具有保护作用。

Objective：to observe the protective effect of Ping Gan Jie Du San on mice with acute liver injury and provide the refer- ence for clinical medicine use. Methods ： CCL4 and TAA were once injected into abdominal cavity of mice to establish the experimental model of acute liver injury. The level of serum ALT and AST and liver histopathology of the mice were examined. Results： CCL4 group ： in the CCL4 and TAA model of acute liver injury, there was a obvious decline of serum ALT and AST and there was less hepatic damage in Ping Gan Jie Du San -treated group using different doses （hing dose, medium dose, low dose）than those not treated with Ping Gan Jie Du San （ p 〈 0. 01 ）. The protective effect of Ping Gan Jie Du San is related to its dosages, it is better to use the high dosage. TAA group ： in the CCL4 and TAA model of acute liver injury, there was a obvious decline of serum ALT and AST and there was less hepatic damage in Ping Gan Jie Du San - treated group using low dose than those not treated with Ping Gan Jie Du San （ p 〈 0. 01 ）.Conclusion：Ping Gan Jie Du San can protect acute liver injury by preventing hepatocyte degeneration and necrosis induced by CCL4 and TAA.