重组Shh因子对海水浸泡肺微血管内皮细胞增殖和凋亡的影响研究

Recombinant sonic hedgehog promotes proliferation and suppresses apoptosis of pulmonary microvascular endothelial cells exposed to seawater

目的观察不同剂量重组Shh(recombinant sonic hedgehog,rShh)因子对海水浸泡肺微血管内皮细胞(pulmonary microvascular endothelial cell,PMVEC)的促进增殖及抗凋亡作用。方法组织块法原代培养大鼠PMVEC,建立大鼠PMVEC海水浸泡模型,加入不同剂量重组Shh因子,采用TUNEL法检测PMVEC凋亡率。采用2,3-苯基溴化四唑(MTT)染色计数法测定细胞增殖活性。结果海水浸泡可导致PMVEC损伤,表现为PMVEC细胞增殖受到抑制以及凋亡发生率增加。重组Shh能降低海水所致PMVEC增殖抑制率和其凋亡率,在实验剂量范围内,其促增殖和抗凋亡作用呈剂量依赖性。结论重组Shh可促进海水浸泡PMVEC增殖和抑制其凋亡。

Objective To observe the effects of recombinant sonic hedgehog （rShh） on the proliferation and apoptosis of cultured pulmonary microvascular endothelial cells （PMVECs） after exposure to seawater. Methods After PMVECs were primarily isolated from SD rats and cultured, and then the cells were exposed to seawater （from the South China Sea） and treated with 50,100,200 and 400 ng/ml rShh respectively for 24 h. Apoptotic rate of PMVECs was detected by TUNEL. The proliferation of PMVECs was examined by MTr assay. Results Seawater caused the proliferation of PMVECs inhibited and the apoptotic rate increased, rShh suppressed the inhibited proliferation and inhibited apoptosis of the cells in a dose-dependent manner. Conclusion RShh can promote the proliferation and inhibit the apoptosis of PMVECs exposed to seawater.