牵张负荷对大鼠膀胱Cajal间质细胞超微形态及c-kit基因表达影响

Effects of stretch load on the ultramicrostructure and c-kit gene expression of the bladder ICCs in rats

目的通过研究膀胱Cajal间质细胞(Interstitial cells of cajal,ICCs)超微形态及c-kit基因水平变化,阐明牵张负荷对膀胱Cajal细胞兴奋性的调控作用。方法结扎膀胱颈,构建大鼠膀胱出口部分梗阻致逼尿肌过度活动模型,通过扫描电镜方法观察牵张负荷条件下逼尿肌过度活动组与正常对照组膀胱ICCs细胞超微形态变化,观察牵张负荷条件下逼尿肌稳定组和逼尿肌过度活动组与正常对照组膀胱ICCs细胞酪氨酸激酶受体(c-kit)在基因水平表达差异。结果逼尿肌过度活动组ICCs细胞形态和c-kit基因水平表达量较正常对照组有明显改变(P<0.01)。结论牵张负荷对ICCs细胞有调控作用,可能的作用机制在于改变ICCs细胞形态及上调c-kit表达。

Objective To investigate the effects of stretch load on the ultramicrostructure and c-kit expression mc the bladder interstitial cells of cajal （ICCs） in rats. Methods Rat model with detrusor overactivity due to partial outflow obstruction with bladder neck ligation were established. The effects of stretch load on the changes of ultramicrostrueture of the bladder ICCs were observed by scanning electron microscopy. The changes in c-kit gene expression of ICCs in stable, overactive, and normal detrusors were analyzed with RT-PCR. Resuits The ultramicrostructure of the ICCs changed significantly. The expression of c-kit was significantly higher in the overactive group than that in stable and normal groups （P 〈 0.01 ）. Conclusion Stretch load can regulate the function of ICCs. The possible mechanism may be associated with the morphological changes in ICCs and up-regulation of c-kit expression.