麻黄碱对K562/A02细胞MDR1、MRP、TopoⅡ和GST表达的影响

Effect of ephedrine on the expression of MDR1、MRP、TopoⅡand GST in K562/A02 Cells

目的:研究麻黄碱对K562/A02细胞株多药耐药性逆转的作用。方法:采用噻唑蓝(MTT)法检测麻黄碱的细胞毒作用;采用半定量逆转录聚合酶链反应(RT-PCR)和免疫印迹分别检测非细胞毒浓度的麻黄碱对K562/A02细胞MDR1、MRP、TopoⅡ以及GST表达的影响。结果:非细胞毒浓度的麻黄碱作用后,K562/A02细胞的MDR1及P-gp表达降低(P<0.01);而TopoⅡ、MRP、GST的表达无明显变化(P>0.05)。结论:麻黄碱能部分逆转K562/A02细胞的耐药性,其作用机制可能与下调K562/A02细胞MDR1的mRNA的表达,导致细胞膜上P-gp的表达量减少有关。

Objective： To explore the reversal effect of ephedrine on human multidrug resistance leukemic cell line K562/A02. Methods ： MTT as- say was used to analyze the eytotoxicity of ephedrine; the semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） and Western Blot were used to detect the mRNA and protein expressiom of MDR1, MRP, Topo Ⅱ , and GST gene of K562/A02 cells after treatment with non-cytotoxic concentration of ephedrine, respectively. Results ： Ephedrine at non-cytotoxic concentration led to significant downregtdation of mRNA and p-glycoprotein expression of MDR1 gene in K562/A02 cells （ P 〈 0.01 ） , but little effect on the no the expression of MRP, Topo Ⅱ , and GST （ P 〉 0.05 ）. Conclusion ： Ephedrine partly reverse multidrug resistance of K562/A02 ceils through downregulating the expression of MDR1 mRNA, which may lead to the reduction of p-glycoprotein expression on celt membrane.