异种化学去细胞神经移植修复大鼠坐骨神经缺损的功能评价

Regeneration of rat sciatic nerve inhanced by chemical acellular xenogeneous nerve grafts:a functional evaluation

[目的]评价异种化学去细胞神经移植修复大鼠坐骨神经缺损后神经功能恢复,从而为临床应用去细胞异种神经移植修复神经缺损提供更为充分的理论依据。[方法]雄性Wistar大鼠15只,致左侧坐骨神经1 cm缺损,以等粗兔化学去细胞神经移植修复。每2周测定坐骨神经指数,移植后4个月动物麻醉后暴露移植段神经,刺激近侧神经干、于同侧胫后肌群记录运动诱发电位,之后取移植段神经切片后行HE组织化学及NF-160免疫组织化学染色。[结果]去细胞异种神经移植物未被宿主排斥,大量神经纤维长入移植物,神经电生理及坐骨神经指数显示宿主坐骨神经功能有部分恢复。[结论]去细胞异种神经移植可以作为修复周围神经缺损的一种有效方法。

[ Objective]To observe the nerve regeneration and functional recovery in the adult rats. [Method] The sciatic nerves on the left side of 15 rats were exposed and 1.0 cm long segments of the nerves were removed from the mid-thigh level and replaced by rabbit nerve made acellular through chemical extraction. At 4 months after procedure, the nerve regeneration and func- tion recovery were examined with HE staining, NF-160 immunohistochemical staining, electrophysiological tests and sciatic functional index （SFI）. [ Result] In the rats repaired by acellular nerves, regenerated axons re-entered into the acellular xeno-nerve segments without excessive sign of inflammation following implantation. As stimuling（ 1.5 mA ,0.1 ms, 1.0 Hz） to the proximal sciatic nerves, the implanted segment resulted in motor evoked potentials, which were recorded from poster/or tibia/ muscles. SFI showed a partial recovery of locomotion of the limb with sciatic nerve defect. [ Conclusion] Sciatic nerves defect can be repaired by chemical acellular xenogeneous nerves in rats. There was partial functional recovery, which revealed the grafting with chemical acellular xenogeneous nerves may be a promising mehod for nerve defect in clinical.