高效液相色谱法测定山楂总黄酮提取物中芦丁和金丝桃苷的含量

Determination of Rutin and Hyperoside in Extract from Crataegus pinnatifida Bge. by HPLC

目的建立高效液相色谱法测定山楂总黄酮提取物中芦丁和金丝桃苷的含量的方法。方法采用HPLC法,色谱柱Kromasil C18色谱柱(4.6mm×250mm,7μm);流动相为甲醇-0.4%磷酸溶液(30∶70),流速1.0mL/min;柱温25℃;检测波长359nm。结果芦丁在0.051μg-0.51μg(r=0.9999)范围内线性关系良好,平均回收率为100.4%(RSD=1.2%);金丝桃苷为0.062μg-0.62μg(r=0.9997)范围内线性关系良好,平均回收率为99.0%(RSD=1.8%)。结论该方法简便、灵敏、可靠、准确、重现性好,可用于山楂提取物的含量测定。

Objective To establish an HPLC method for Determination of hypereside and rutin in the extract from Grataegus pinmuifida Bge. Method HPLC was used in the quantitative analysis by using Kremasil Cis （4. 6mm × 250mm, 7μm）chromatography column and methanol -0.4% phosphoric acid（30：70） as a mobile phase. The flow rate of mobile phase was 1.0 mL/min. The detection wavelength was at 359nm. Result The linear ranges for rutin and hypereside were 0. 051 -0.51μg （r =0. 9999） ,0. 062 -0. 62μg （ r =0.9997）, respectively. The average recoveries were 100.4% （RSD = 1.2% ）, 99. 0% （RSD = 1.8% ）, respectively. Conclusion This method is easy, sensitive, reliable, accurate, reproducible and can be used to determine the contents of hyperoside and rutin in the extract from Grataegus pinnatifida Bge..