摘要
在已经定位的QTL区域中开展基因连锁定位是寻找候选基因的重要途径.运用比较基因组方法筛选出猪2号染色体上生长、胴体和肉质性状QTL区域中与肌肉发育相关的5个基因(MYOD1、LDHA、CSRP3、TEF-1和COPB1),通过RH和连锁定位方法研究这5个基因的排列顺序和距离,并与人和小鼠基因的顺序进行了比较.结果表明这5个基因在猪2号染色体上的排列顺序和距离是:MYOD1(75.2 c M)-LDHA(79 c M)-CSRP3(83.8 c M)-TEF-1(86.5 c M)-COPB1(90 c M),猪与小鼠基因的顺序相同,而与人(TEF-1-COPB1-MYOD1-LDHA-CSRP3)的顺序不一致.本研究结果对于进一步开展这5个基因的功能研究奠定了基础.
Developing linkage mapping of genes in the QTL regions, which have been mapped, is the important way to find candidate genes. In this paper, MYOD1, LDHA, CSRP3, TEF-1 and COPB1 genes were selected in the growth, carcass and meat quality QTL region of pig chromosome 2 by comparative genome method. The order of the five genes was determined through RH and linkage mapping, and compared with human and mouse. The results showed that the order of the five genes in pig chromosome 2 was： MYOD1 （75.2 cM）-LDHA （79 cM）-CSRP3 （83. 8 cM）-TEF-1 （86. 5 cM）- COPB1 （90 cM）. The order of pig is the same as mouse, but different with human （TEF-I-COPBI- MYOD1-LDHA-CSRP3）. This study laid a foundation for further function study of these five genes.
出处
《湖北大学学报（自然科学版）》
CAS
北大核心
2009年第2期172-175,188,共5页
Journal of Hubei University：Natural Science
基金
国家自然科学基金(30771536)资助
