RP-HPLC法同时测定复方甘草酸苷片中三组分的含量

Determination of Three Kinds of Components in Compound Glycyrrhizin Tablet by RP-HPLC

目的:建立高效液相色谱法同时测定复方甘草酸苷片中甘草酸单铵盐、甘氨酸和蛋氨酸的含量。方法:采用Diamonsil C18色谱柱(150mm×4.6mm,5μm),流动相为乙腈-0.01mol·L-1磷酸溶液(40:60),流速1.0mL·min-1,甘草酸单铵的检测波长为257nm,甘氨酸和蛋氨酸的检测波长为262nm。结果:甘草酸单铵盐、甘氨酸和蛋氨酸浓度分别在50.52～505.20μg·mL-1、10.6～106.0μg·mL-1、10.4～104.0μg·mL-1范围内呈良好的线性关系;平均回收率分别为(100.32±1.35)%、(101.02±1.80)%和(100.75±1.86)%,RSD分别为1.35%、1.78%和1.85%(n=9)。结论:本法可用于同时测定复方甘草酸苷片中甘草酸单铵盐、甘氨酸和蛋氨酸的含量,方法简便,结果准确。

Objective： To establish the RP-HPLC method for determination of ammonium glycyrrhizinate, glycine and methionine in compound glycyrrhizin tablet. Methods： The column C18 （150 mm × 4.6 mm, 5 μm） was used. The mobile phase consisted of aeetonitrile-0.01 mol ·L^-1 phosphate solution （40 ： 60）. The flow rate was 1.0 mL · min^-1, and the detection wavelength were 257 nm and 262 nm respectively. Results： A good linear range of 50.52 - 505.20 μg·mL^-1 for ammonium glycyrrhizinate, 10.6 - 106.0 μg · mL^-1 for glycine and 10.4 - 104.0 μg·mL^-1 for methionine were showed respectively. The recoveries of glycyrrhizin, glycine and methionine were （100.32 ± 1.35）% （RSD = 1.35%）, （101.02 ±1.80）% （RSD = 1.78%） and （100.75±1.86）% （RSD = 1.85%） respectively. Conclusion： The method is accurate for determining glycyrrhizin, glycine and methionine in compound glycyrrhizin tablet.