胎膜早破与细胞凋亡的相关性研究

Correlative study on premature ruptute of fetal membranes and apoptosis.

目的探讨细胞凋亡增加是否为胎膜早破的一个发病机理或危险因素。方法随机选择经阴道分娩的足月初产妇100例的胎膜，其中胎膜早破者50例作为研究组，其他50例为对照组，用免疫纵化法检测两组胎膜组织中Bax、Bcl-2、细胞色素c的表达情况。结果胎膜早破组促凋亡蛋白Bax、细胞色素C的阳性表达率高于对照组（P〈0．005），但抑凋亡蛋白Bcl-2阳性表达率两组间无统计学差异（P〉0．5）。结论胎膜早破的发生与促凋亡蛋白Bax，Cyt—c表达的增强相关，基因调控下胎膜细胞凋广增加可能是胎膜早破的一个重要的发病机制。

Objective To explore whether the increased apoptosis in fetal mombranes is a pathogenesis or a risk factor of premature rupture of membranes（PROM）. Methods Randomize fetal membranes samples were collected from 100 childbirth women through vanina, including 50 cases of PROM and 50 cases others as controls, the expressions of Bax, Bcl - 2, Cytochrome - c in fetal membrages were determined by immunohistochemistry. Results The positive expression rates of proapoptotic - proteins Bax, cytochrome - c in fetal membranes of the PROM group were sinnificantly higher than control group（ P 〈 0. 005 ）, whereas antlapoptotic -protein Bcl -2 expressi on between two groups was not significant（P 〉 0. 05 ）. Conclusion These result suggest that the increase of Bax, Cytochrome - c expression can remarkably induce apoptosis and promote PROM, and increased apoptosis in membranes by gene - enviroment may be one of the means in the pathogenesis of PROM.