RP-HPLC法测定番石榴叶中桑黄素来苏糖苷和桑黄素阿拉伯糖苷的含量

RP-HPLC Determination of Morin-3-O-α-L-lyxopyranoside and Morin-3-O-α-L-arabopyranoside from Psidium guajava leaf

目的:建立番石榴叶中桑黄素来苏糖苷和桑黄素阿拉伯糖苷的含量测定方法。方法:用Diamonsil C18色谱柱(250mm×4.6mm,5μm),以甲醇-0.1%磷酸溶液(35∶65)为流动相,流速为1.0mL·min-1,检测波长为355nm。结果:桑黄素来苏糖苷在0.06132～0.14308μg范围内呈良好的线性关系(r=0.9999);桑黄素阿拉伯糖苷在0.05844～0.13636μg范围内呈良好的线性关系(r=1),平均回收率分别为98.6%和100.7%,RSD分别为1.0%和1.7%。结论:本方法对番石榴叶质量标准的制定具有很好的参考价值。

Objective： To develop a RP-HPLC method for determination of Morin-3-O-α-L-lyxopyranoside and Morin-3-O-α-L-arabopyranoside in Psidium guajava L.. Methods： A Diamonsil C18 column （250 mm × 4.6 mm,5μm） was used, methanol-0.1% phosphoric acid solution （35：65）as the mobile phase, flow rate at 1.0 mL· min^-1, and the detection wavelength was 355 nm. Results： The of calibration curve Morin-3-O-α-L-lyxopyranoside was linear between 0.061 32-0.143 08 μg（ r = 0.999 9）, The calibration curves of Morin-3-O-α-L-arabopyranoside was linear between 0.058 44 - 0. 136 36 μg（ r = 1 ）, The average recoveries were 98.6 % and 100.7 % , The relative standard deviations were 1.0% and 1.7%. Conclusion： The method could be used for determination of Morin-3-O-α-L-lyxopyranoside and Morin- 3-O-α-L-arabopyranoside from Psidium guajava L..