摘要
本实验采用反相高效液相色谱法实现了对乳酸、乳酸甲酯的分离并对其定量分析效果进行研究。HPLC分析条件为:Purospher STAR C18反相柱,二极管阵列检测器,以0.005mol/LH2SO4与甲醇混合溶液(9:1,V/V)为流动相,流速为1.0ml/min,进样量20μl,检测波长210nm。该方法快速、简便、灵敏、重现性好。
A reversed-phase high performance liquid chromatographic （RP-HPLC） method was developed for the simultaneous determination of lactic acid and methyl lactate. The HPLC was equipped with Purospher STAR Ci8 reversed-phase column and diode array detector. The mobile phase was composed of 0.005 mol/L H2SO4 solution and chromatographically pure methanol （9：1, V/V） with the flow rate of 1.0 ml/min. The injection volume was 20μl, and the detection wavelength was set as 210 nm. This method is accurate, simple, rapid and reproducible.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第12期165-167,共3页
Food Science
基金
国家"863"计划项目(2007AA10Z361)
