摘要
建立超高效液相色谱串联质谱法(UPLC-MS/MS)测定苹果中苯并咪唑类残留的快速检测方法。样品经乙腈提取后盐析,过0.2μm滤膜,电喷雾电离,正离子模式,多反应检测(MRM)。多菌灵、噻菌灵、甲基硫菌灵的检出限分别为0.17、0.16、0.07μg/kg,添加回收率的范围89.2%～104.6%,相对标准偏差为1.7%～3.6%。本方法快速、易操作、灵敏度高,完全满足农药残留检测的需要。
Objective： To establish a method for determination of benzimidazoles residues in apples by UPLC-MS/MS. Methods： Sample was extracted with acetonitrile. After impurity removal by NaCI salting out, the sample was filtered through a membrane （0.2/am）, and injected into UPLC column. Identification was achieved by electrospray ionization in positive mode （ESP） using multiple reaction monitoring （MRM）. Results： The detection limits for carbendazim, thiabendazol, thiophanatemethyle were 0.17, 0.16, 0.07 μg/kg, respectively; the average recoveries for the three benzimidazoles in blank sample spiked at three levels （1.0, 5.0 and 10.0μg/kg） were in the range of 89.2%- 104.6%, and the RSDs were 1.7%-3.6% （n = 6）. Conclusion： The method can completely meet the requirement of analysis for benzimidazoles residues in apples.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第12期209-212,共4页
Food Science
