山药粗多糖脱蛋白方法的对比研究

Study on the comparison of different methods for removing of protein from polysaccharide of Chinese yam

采用水提醇沉法提取山药粗多糖,采用三氯乙酸(TCA)法、盐酸法、胰蛋白酶(TPS)法对山药多糖进行脱蛋白研究。结果表明,胰蛋白酶法可以有效脱除蛋白并使多糖损失较小;其最佳条件为胰蛋白酶用量0.5 mL、酶解时间45 min、酶解温度为37℃,在该条件下,蛋白脱除率为88.7%,多糖保留率为93.1%。

Extracting polysaccharide with the method of decoction and alcohol sedimentation. The different ways to removing protein from polysaccharJde of Chinese yam among TCA, HCI and TPS is compared. The result shows TPS method is the best way to removing protein and can remove the protein effectively and lose polysaccharide little, the best condition is keeping the quantity of TPS 0.5 mL, decomposing time 45 min and decomposing temperature 37 ℃. The removing rate for protein is 88.7% and retaining rate for polysaccharide Js 93.1%.