钳夹法制备急性眼外肌损伤模型

Establishment of an animal model for acute extraocular muscle injury with clamping

目的:探讨用钳夹法制备急性眼外肌损伤模型,为研究急性眼外肌麻痹的机制和治疗方法提供可应用的实验模型。方法:成年雄性新西兰白兔24只,随机分为A,B,C3组,每组8只。左眼常规消毒后,打开结膜囊,暴露上直肌,以20cm反向钳(钳夹力约1200g)分别在左眼上直肌近附着点处钳夹5min(A),10min(B),20min(C)作为实验组,右眼上直肌不做任何处理作为对照组(D)。术后于第7d处死兔子,每组各取8条肌肉HE染色组织病理学观察。结果:组织病理学观察发现,实验A,B,C各组上直肌较对照组有不同程度的肌纤维断裂、坏死和肌浆融解,在40×10光镜下横切面肌纤维计数结果(个/400倍光镜视野)分别为:A:45.25±3.54,B:34.00±3.53,C:17.83±3.19,D:61.25±4.89,实验各组肌纤维数量较对照组分别减少26.12%(A,P<0.05),44.49%(B,P<0.01),71.76%(C,P<0.01),钳夹时间越长损伤程度越严重,5,10,20min组损伤程度分别符合肌肉损伤Ⅱ轻度、中度、重度标准。结论:钳夹法可以导致眼外肌急性损伤,可作为急性眼外肌损伤模型的制作方法。

AIM ：To establish an animal model for acute paralysis of extraocular muscles. METHODES： Forty-eight eyes of 24 adult male New Zealand albino rabbits were randomly divided into A, B, C groups（8 rabbits in each group）. Open the conjunctiva of the left eye after the conventional disinfection to expose the superior rectus, then clamp the superior rectus by needle of 20 cm （clamping force： 1 200g） holder for 5 minutes （A）, 10 minutes （B）, and 20 minutes （C）, respectively. And there was no treatment in the right eye （control group：D）. All rabbits were sacrificed on the 7th day after the operation. Then the histopathology changes after HE dyeing were observed. RESULTS： Histopathology observation showed that muscle fiber necrosis, fragmentation and cytoplasmic lysis had happened in the superior rectus of the experimental groups in different extent. Results（/400 light microscope field） of transaction muscle fiber counting at 40 × 10 light microscope showed ： A：45.25 ± 3.54 ; B ：34.00 ± 3.53; C ：17. 83 ± 3.19 ; control group：61.25±4.89. Quantity of muscle fibers counting decreased than control group： 26.12%（A, P〈0.05）, 44.49%（B, P〈0.01）, 71.76%（C, P〈 0.01）. The histopathology change after 5, 10, 20 minutes clamping were accorded with muscle injury： Ⅱ low-grade, midrange and heavy-grade, respectively. CONCLUSION ： Clamping extraocular muscle can induce the acute injury, which can be used for the establishment of an animal model for acute