IL-13基因多态性与RSV感染后婴幼儿喘息及哮喘关系的研究

The relationship between IL-13 gene polymorphism and wheezing post RSV infection in infants and asthma

目的探讨IL-13基因Arg110Gln多态性在RSV感染后婴幼儿喘息发生和哮喘发病中的作用。方法哮喘患儿和正常儿童各96例,RSV感染后喘息婴幼儿112例,取其颊黏膜细胞,提取DNA,用实时PCR法对IL-13Arg110Gln位点进行基因分型。结果IL-13Arg110Gln位点的基因型和等位基因频率分布在哮喘组和对照组间差异有高度统计学意义(P<0.01);哮喘组的A等位基因和A/A纯合子基因型频率明显高于对照组(分别为68.2%和47.4%,46.9%和29.2%,P<0.01)。IL-13Arg110Gln位点的基因型和等位基因频率分布在哮喘组和RSV感染后喘息组间差异无统计学意义(P>0.05)。结论IL-13Arg110Gln位点G→A变异与RSV感染后婴幼儿喘息发生和哮喘发病相关,两者在该位点上可能存在相似的遗传基础。

Objective To explore the effects of IL-13 gene Arg 110 Gin polymorphism on asthma and wheezing post RSV infection in infants. Methods Ninety-six 96 children with asthma and 112 wheezy infants were enrolled in the study. All subjects were previously infected by RSV. Ninety-six healthy children were studied as control. DNA was extracted from buccal mucosa cells and real-time PCR analysis was applied to detect IL-13 gene Arg 110 Gin polymorphism. Results Notable diversity was found in genotype and allele frequency distribution between asthma and control groups （P 〈 0.01 ） . The frequency of allele A and genotype A/A in asthma group was significantly higher than those in the controls （68.2% versus 47.4% and 46.9% versus 29.2%, respectively）. There was no statistical differences in genotype and allele frequency distribution between wheezing and asthma groups （P 〉 0.05）. Conclusions G→A variation of IL-13 Arg 110 Gin locus were associated with the development of infant wheezing and asthma post RSV infection, and the two diseases may have similar genetic etiology at this locus.