AtNHX1基因对菊苣的转化和耐盐性研究

Transformation of Cichorium intybus with the AtNHX1 gene and salinity tolerance of the transformants

用农杆菌介导法将AtNHX1基因导入菊苣中,共获得42株卡那霉素(Kan)抗性再生植株。经过PCR检测、Southern杂交和RT-PCR检测表明,AtNHX1基因已成功整合到菊苣基因组中,并且能够正常转录。野生型和转基因植株诱发的愈伤组织进行耐盐生长试验,结果显示,相同盐胁迫条件下,转基因愈伤组织的相对生长率显著高于野生型愈伤组织。施加梯度NaCl胁迫后,植株叶片K+和Na+含量测定结果显示,转基因植株叶片比野生型积累更多的Na+和K+,维持较高的K+/Na+;叶片相对电导率测定结果表明,转基因株系叶片相对电导率显著低于野生型。上述结果表明,AtNHX1基因的导入和表达在提高菊苣耐盐性的同时减轻了盐胁迫对植物细胞膜的伤害。

The AtNHX1 gene was transferred into Cichorium intybus using Agrobacterium and 42 regenerated plants resistant to Kanamyein （Kan） were obtained. Incorporation and expression of the AtNHX1 gene in the transformants were confirmed by PCR, Southern blot, and RT-PCR. Under the same salt stress conditions, the transgenic calli with NaCl resistance, that were induced from transgenic plants, had considerably higher relative growth rates than the wild-type calli. Under the stress of different concentrations of NaCl, the K^＋/ Na^＋ ratio in the transgenie plant cells was always higher than that in the wild-type, whereas the relative conductivity was the opposite. The transformation of the AtNHX1 gene not only enhanced the salt tolerance of transgenic C. intybus, but also reduced ceil membrane damage induced by salinity.