IVF/ICSI受精失败的原因

The causes of IVF/ICSI fertilization failure

全部卵母细胞受精失败（或低受精率）是体外受精（IVF）实验室经常遇到的困惑之一。尽管在熟练掌握和广泛使用卵胞浆内单精子注射（ICSI）技术后，全卵不受精现象已显著降低，但在IVF实验室内仍然存在着难以预测和不明原因的全卵不受精现象。事实上，无论是采取常规IVF或ICSI助受精方式，都避免不了某些病例出现全卵受精失败。

Objective：The purpose of this study was to analyze the causes of in vitro fertilization failure in a university-affiliated assisted reproductive medical center. Method： Retrospective analysis of clinical materials. Results： A total of 4,566 IVF treatment cycles （2,883 conventional IVF and 1,683 ICSI） were performed in 2008. There were 154 cases of fertilization failure（fertilization rate 420%）, representing an incidence of 3.37 %. Among them, 31 cases （31/1,683, 1.84 %） of fertilization failure occurred after ICSI,and 123 cases （123/2,883, 4.27 %） of fertilization failure occurred in conventional IVF cycles. Only 77 cases can be rescued by late ICSI after conventional IVF failure. Conclusion：Most of the fertilization failures can be attributed to inadequate numbers of oocytes and their poor qualities.