补肾抗衰汤对氧化应激所致的秀丽隐杆线虫生育缺陷的影响(英文)

Effects of Bushen Kangshuai Tang in retrieving oxidative stress-induced reproductive defects in Caenorhabditis elegans

目的:探讨补肾抗衰汤对秀丽隐杆线虫因氧化应激诱导所致生育缺陷的作用。方法:将终浓度为0.33g/mL的补肾抗衰汤按25%、50%、75%和100%梯度稀释后对L4期线虫培养。应激方式采用紫外线照射[20J/(m2.min)]、热休克(36℃,2h)和百草枯(2mmol/L,2h)处理。通过子宫内卵数、卵的大小和传代时间来评价秀丽隐杆线虫的生育能力。结果:野生型N2线虫经过紫外线照射、热休克和百草枯处理后,子宫内卵数、卵的大小均显著减少,传代时间明显增加;过氧化氢酶和过氧化物歧化酶活性被抑制。补肾抗衰汤对野生型线虫没有毒性或其他副作用。高浓度补肾抗衰汤不仅能够减轻由紫外线照射、热休克和百草枯所导致的生育缺陷,而且可以增加过氧化氢酶和超氧化物歧化酶被各种应激抑制的活性。此外,高浓度补肾抗衰汤可以明显恢复mev-1突变体线虫受损的生育能力。结论:氧化应激对生育能力有负面影响,高浓度补肾抗衰汤可明显逆转氧化应激所致的线虫生育缺陷。

Objective： To explore the function of Bushen Kangshuai Tang （BKT）, a compound traditional Chinese herbal medicine, in alleviating oxidative stress-induced reproductive defects in organism nematode Caenorhabditis elegans . Methods： The L4-1arvae were cultured with 25%, 50%, 75%, and 100% of BKT with the final concentration of 0.33 g/mL. Ultraviolet irradiation [20 J/（m^2.min）], heat-shock （36 ℃, 2 h）, and paraquat treatment （2 mmol/L, 2 h） were exerted as the stresses. Reproduction was assayed by the egg number in uterus, brood size, and generation time. Results： Ultraviolet irradiation, heat-shock, and paraquat treatment could significantly reduce egg number in uterus and brood size, increase generation time, and suppress activities of catalase and superoxide dismutase of the treated wild-type N2 nematodes. Administration of BKT did not cause toxic or altered effects on reproduction under wild-type background in C. elegans. However, BKT administration at higher concentrations could not only effectively alleviate the reproductive defects induced by ultraviolet irradiation, heat-shock, and paraquat treatment, but also increase the catalase and superoxide dismutase activities suppressed by ultraviolet irradiation, heat-shock, and paraquat treatment as compared with the control. Moreover, administration of BKT at higher concentrations could largely recover the reproductive defects formed in mev-1 mutant nematodes with elevated oxidative stress. Conclusion： Oxidative stress can negatively regulate the reproductive process, and the administration of high concentrations of BKT will largely retrieve the oxidative stress-induced reproductive defects in exposed nematodes.