凉血解毒方治疗银屑病的实验研究

Effects of Liangxue Jiedu Decoction in treating psoriasis in a mouse psoriasis model

目的:观察凉血解毒方对小鼠尾部有颗粒层的鳞片和阴道上皮细胞有丝分裂的影响,探讨其治疗银屑病的可能作用机制。方法:选取60只小鼠随机分为凉血解毒方组、复方青黛胶囊组、阿维A胶囊组和生理盐水组,每组15只,另选10只正常小鼠作为空白对照组。按人鼠等效剂量连续灌胃相应药物14d,1次/d,第15天处死,取材,苏木精和伊红(hematoxylinandeosin,HE)染色法检测并计算尾部表皮细胞中颗粒细胞百分比。随机选取60只雌性小鼠,腹腔注射乙烯雌酚3d后分为上述4组,继续腹腔注射乙烯雌酚。另选用10只正常小鼠作为对照组。分组和灌胃方法同前。第15天小鼠腹腔注射秋水仙碱(2mg/kg),6h后处死,取材,HE染色法观察并计算小鼠阴道上皮细胞有丝分裂指数。结果:与生理盐水组比较,凉血解毒方组小鼠尾部鳞片颗粒细胞增加(P<0.01),小鼠阴道上皮基底细胞的有丝分裂细胞数减少(P<0.01)。结论:凉血解毒方能促进小鼠尾部颗粒层形成和显著抑制小鼠阴道上皮细胞的有丝分裂,其治疗银屑病的机制可能与促进表皮细胞正常分化和抑制表皮细胞增殖有关。

Objective： To study the effects of Liangxue Jiedu Decoction, a compound traditional Chinese herbal medicine with the function of blood-cooling and detoxicating, in treating psoriasis in mice and to explore its mechanism. Methods： （1） Sixty mice were randomly divided into Liangxue Jiedu Decoction group, compound Indigo Naturalis capsule group, acitretin capsule group and normal saline group. Another 10 mice were selected as blank control. After 2-week administration, mice were sacrificed to obtain samples. After hematoxylin and eosin （HE） staining, tail scales with granular layers were calculated by an optical microscope. （2） Except for ten mice in blank group, sixty female mice were injected intraperitoneally with diethylstilbestrol once daily. After 3-day injection, mice were randomly divided into four groups and treated as above description. After 2- week treatment, all mice were injected intraperitoneally with colchicine （2 mg/kg）, and sacrificed 6 h after the injection. The mitotic rate in virginal epithelium was calculated after HE staining. Results： Compared with normal saline, Liangxue Jiedu Decoction could significantly inhibit the mitosis of mouse vaginal epithelium （P〈0. 01） and promote the formation of granular layers in mouse tail-scale epidermis （P〈0.01）. Conclusion： The mechanism of Liangxue Jiedu Decoction in treating psoriasis may be related to promoting granular cell growth and inhibiting proliferation of epidermic cells .