啤酒酵母培养条件对ATP生产的影响 Ⅰ.供氧对酵母合成ATP活性的影响

Effects of Culture Conditions on ATP Production by Brewer's Yeast 

结合酵母细胞酶促合成ＡＴＰ的活性测试，在５Ｌ发酵罐中培养啤酒酵母，考察了通气对菌体生长和细胞活性的影响。实验结果表明：通气量的变化，对菌体的生长速度和得率具有明显的调节作用；采用氧限制方法培养的啤酒酵母用于ＡＴＰ生产时得率可达到１００％。通气量６．０Ｌ／（Ｌ·ｈ）时培养的酵母细胞用于ＡＴＰ生产的效率比在通气量６０Ｌ／（Ｌ·ｈ）下培养的酵母细胞提高５０％以上。

The effect of aeration rate was studied in the culture of brewer's yeast used for ATP production. Under aerated condition, the yeast growth rate and growth yield were enhanced. The yeast cell activity for the ATP production increased at oxygenlimited conditions. These results indicate that aeration plays an important role in yeast metabolism and physiology.