厄贝沙坦对糖尿病大鼠肾脏GluT-1及TGF-β1表达的影响及其临床意义

Effect of irbesartan on mRNA expression of GLUT-1 and TGFβ-1 in the kidney of diabetic rats and the clinical significance

目的观察厄贝沙坦(IBST)对糖尿病大鼠肾脏皮质葡萄糖转运蛋白-1(GluT-1)及转化生长因子-β1(TGF-β1)表达的影响,并探讨其作用机制。方法 48只大鼠随机分为对照(Con)组、糖尿病(DM)组与IBST治疗组,建立糖尿病肾病(DN)模型,分别检测肾皮质GluT-1 mRNA及TGF-β1 mRNA表达水平。结果与Con组相比,DM组大鼠肾组织GluT-1 mRNA表达及TGF-β1 mRNA表达均显著上调(P<0.01)。厄贝沙坦干预后,IBST组肾组织GluT-1及TGF-β1 mRNA表达显著低于DM组(P<0.01)。结论厄贝沙坦对DN有保护作用,这可能与厄贝沙坦显著降低肾组织G1uT-1及TGF-β1 mRNA表达有关。

Objective To investigate the effect of irbesartan, on the mRNA expressions of GluT-1 and TGF-β1 in the kidney of diabetic rats, and investigate their clincal significance, Methods The 34 STZ-indnced diabetic Wistar rats were divided into simple diabetes（group B,n= 17） and irbesartan-treated diabetes （group C,n= 17） ,and 14 healthy rats were used as control （group A）. The body weight,plasma glucose,serum creatinine,blood urea nitrogen,cholesterol,triglyceried,24-hour urinary protein excretion were measured in each rat. The renal tissues were examined histologically by HE staining and PAS staining. Renal mRNA expression of GluT-1 and TGF-β1 were detected by RT-PCIL Results The plasma glucose,serum creatinine,blood urea nitrogen,cholesterol,triglyceried, 24-hour urinary protin excretion, renal rnRNA expression of GluT-1 and TGF-β1 were increased significantly in group B versus group A. （P〈0.01）. 24-hour urinary protein excretion, serum creatinine and blood urea nitrogen were decreased significantly in gruop C versus group B. Renal mRNA expression of GluT-1 and TGF-β1 decreased significantly （P〈0. 01）. Conclusions Irbesartan can prevent the injury of renal function in STZ-induced diabetic rats. The mechanism might be related to the suppression of expression of GluT- 1 and TGF-β1 mRNA.