基于分子生物学和基因组学的植物根际微生物研究

Study of Rhizosphere Microbe Based on Molecular Biology and Genomics

根际微生物是指在植物根系直接影响的土壤范围内生长繁殖的微生物。根际微生物研究对微生物生态学和工业生物技术开发均十分重要,妨碍其研究进展的主要原因是99%的根际微生物物种在实验室中无法成功培养。近年来,将基于分子生物学和基因组学的非培养研究技术应用于根际微生物多样性研究取得了很大进展,本文结合作者对红豆杉根际的研究,评述近期相关新技术的应用和其未来应用前景。

The rhizosphere is the narrow region of soil that is directly influenced by roots and associated soil microorganisms. Research on rhizosphere microbe is essential for microbial ecology and industrial biotechnology. However, the inability of culturing most rhizosphere microorganisms （around 99%） in the laboratory obviates the research progress. In recent years, there is enormous advance in applying non-culturing techniques based on molecular biology and genomics to the study of rhizosphere microbial diversity and plant-microbe interactions. This review discusses recent findings and future challenges in the study of rhizosphere microbe, with brief comment of our Taxus rhizosphere study and various novel techniques.