四氮戊省磺酸钠对半乳糖性白内障防治及其醛糖还原酶抑制作用

Sodium 5,12-dihydro 5,7,12,14-azapentacene disulfonate alleviates galactose-induced cataract in rats and inhibits aldose raductase activity

目的:考察本实验室自制四氮戊省磺酸钠对半乳糖性白内障的防治效果及其对醛糖还原酶(AR)的抑制作用。方法:造大鼠半乳糖性白内障模型,同时给予高、低浓度药物滴眼液治疗,并以市售法可林滴眼液作阳性对照。观察大鼠白内障生成情况;检测各组大鼠晶状体中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)等活性和丙二醛(MDA)含量;用饱和硫酸铵盐析法提取牛晶状体中的AR,测定四氮戊省磺酸钠对其抑制作用。结果:在考察四氮戊省磺酸钠对大鼠半乳糖性白内障的影响实验中,药物治疗组大鼠的白内障发展情况缓慢于未给药的模型对照组,且高浓度组缓慢于低浓度组。药物能有效增强晶状体中SOD、GSH-PX、CAT活性(P<0.01,P<0.01,P<0.05),降低MDA含量(P<0.05)。同时四氮戊省磺酸钠对于醛糖还原酶具有良好的抑制作用。结论:四氮戊省磺酸钠对于大鼠半乳糖糖性白内障具有确切的防治作用,并对醛糖还原酶也具有良好的抑制作用。

Objective ： To investigate the effects of sodium 5,12-dihydro 5,7,12,14-azapentacene disulfonate on galactose-induced cataract in rats and on the activity of aldose reductase （AR）. Methods： Cataract was induced by galactose in rats, and treated by azapentacene disulfonate eye-drops at lower or higher concentration with commercial Phacolin as the control. The activities of superoxide dismutase （SOD） , glutathione peroxidase （ GSH- PX） and catalase （CAT） as well as malondialdehyde （MDA） content in the lens were measured. AR was extracted from bovine lens with saturated ammonium sulfate, and its inhibition by azapentacene disulfonate was determined. Results： After treatment with azapentacene disulfonate eye-drops, the lens opacities was attenuated, the activities of SOD, GSH-PX and CAT were significantly increased （P 〈 0.05 - 0.01 ） , and MDA content was reduced （P 〈 0.05） in rats with galactose-induced cataract. Aazapentaeene disulfonate also inhibited aldose reductase activity. Conclusion： The synthetic azapentacene disulfonate can delay and alleviate aglactose-induced cataract of rats, and can inhibit aldose reductase in bovine lens.