不同日龄大白猪视黄酸受体α基因组织表达

Research on expression of landrace RARα gene on different days

研究采用RT-PCR方法对大白猪的视黄酸受体α基因在1日龄、90日龄、180日龄、270日龄和360日龄的心、肝、胃、脾、肾、肺、大肠、小肠、肌肉、子宫、卵巢共11个组织的表达情况进行了研究。结果表明,RARαmRNA在肝、脾、肾、大肠、小肠、子宫和卵巢中持续表达,其中脾、大肠和小肠是持续高表达;180日龄时,所有组织的RARαmRNA的表达量普遍降低;360日龄时,所检的11个组织均高水平表达该基因。

Expression level of RARα gene in landrace heart, liver, stomach, spleen, kidney, lung, large intestine, small intestine, muscle, uterus and ovary on 1d, 90d, 180d, 270d and 360d were studied by RT-PCR method. The result showed that RARa mRNA was continuously expressed in liver, spleen, kidney, large intestine, small intestine, uterus and ovary. Among those, the spleen, large intestine and small intestine were continuous high level RARα mRNA expression. The expression level of all tissues RARα mRNA was all reduced on 180d, but it was high expression in all tissues on 360d.