超声-微波协同萃取-HPLC检测掺罂粟壳肉汤中的生物碱

Determination of the Alkaloid in Food Added Opium Poppy Husk by Ultrasonic-microwave Synergistic Extraction-HPLC

为建立食品中可能出现的罂粟壳生物碱成分——吗啡、可待因、罂粟碱的提取和测定方法,用超声-微波协同萃取的方法提取罂粟壳中的生物碱,高效液相色谱法(HPLC)进行含量分析.其平均回收率分别为97.87%,96.80%,94.47%;精密度相对标准偏差(RSD)分别为0.070%,0.037%,0.029%;重现性相对标准偏差(RSD)分别为0.040%,0.209%,0.665%.本方法具有回收率高、灵敏度高、精密度高等优点,可适用于食品中罂粟壳生物碱的检测.

A method for the extraction and determination of morphine,codeine and papaverine in opium poppy husk probably added in foods was developed. The alkaloid in opium poppy husk were extracted by ultrasonic-microwave synergistic extraction technique and determined by high performance liquid chromatography （HPLC）. The average recovery are 97. 87%,96.80% ,94.47% and the RSD of the precision are 0.070% ,0. 037% ,0. 029% and the RSD of the reproducibility are 0. 040% ,0. 209% ,0. 665%0, respectively. The method had high recovery, sensitivity and precision, that is suitable for the determination of the alkaloid in opium poppy husk in food.