摘要
目的:从玉郎伞中分离出玉郎伞多糖,并对其化学组成进行研究。方法:玉郎伞经醇提后的药渣用热水提取,乙醇沉淀,Sevag试剂脱蛋白,采用DEAE纤维素(DEAE-52)柱层析分离纯化,气相色谱和薄层色谱分析其组成。结果:分离得到的玉郎伞多糖经Sephadex-75凝胶柱层析,硫酸-苯酚法以及高效凝胶渗透色谱法(HPGPC)证实为单一多糖,紫外扫描显示无核酸和蛋白特征吸收峰,薄层色谱和气相色谱表明由葡萄糖和阿拉伯糖组成。结论:玉郎伞多糖首次从玉郎伞中提取获得,为玉郎伞主要成分之一。
AIM ： To study the isolation and composition of Cliffbean （ Millettia pulchra kurz） polysaccharide. METHODS： Cliffbean was extracted by boiling water. The polysaccharide in the filtrate was precipitated fraction- ally by alcohol. The protein in the product was removed by Sevag method, and was further purified by DEAE ion- exchange cellulose（ DEAE-52 ）. The component of the saccharide was determined by GC and TLC. RESULTS： The polysaccharide obtained in this way didn＇ t contain protein and nucleic acid. It showed a single polysaccharide when identified by means of Sephadex G-75 column or High Performance Gel Permeation Chromatography （ HPG- PC）. It was composed of glucose and arabinose. CONCLUSION： Cliffbean polysaccharide was first isolated from its root and was one of the important components.
出处
《中成药》
CAS
CSCD
北大核心
2009年第6期903-905,共3页
Chinese Traditional Patent Medicine
基金
广西科学研究与技术开发计划项目(桂科攻0630002-2A)
