血型物质和巯基乙醇中和完全抗体后IgG抗体效价比较

Comparison of the two methods of complete antibody neutrolization during the prenatal examination in patients with neonatal hemolytic disease

目的探讨两种方法中和孕妇血液中血型完全抗体后，免疫性抗-A（IgG）抗体效价临界值。方法选择丈夫为A型、孕妇为O型的抗-A（IgG）抗体效价为1：（4-1024）血液标本50份，经血型物质和巯基乙醇（2-Me）中和完全抗体后采用抗人球蛋白法检测抗-A（IgG）抗体效价。结果血型物质和2-Me两种方法中和完全抗体后测得抗-A（IgG）抗体效价均数分别为22．01和36．25，差异有统计学意义（P〈0．001）。孕妇血液经血型物质和2-Me两种方法中和完全抗体后IgG抗体效价临界值应分别设定为1：16和1：64。结论血型物质和2-Me中和完全抗体的能力有差异，因此进行新生儿溶血病检查时应注意采用不同临界值。

Objective To understand the two methods of the neutrolization of the complete antibodies in pregnant women,and cmparise the immunological anti-A（IgA） titer. Methods Blood type material neutrolization method, 2-mercaptoethanol （2-Me） neutrolization and anti-human globulin method were employed to detect anti-A IgG titer. Results After the antibodies were neutrolized by the two methods, the titer of anti-A IgG was determined by anti-human globulin method. There was obvious difference （P〈 0.01） by statistical analysis. Conclusion When the prenatal IgG titer examined by neutrolization with blood type material in the pregnant women reached 1 ： 16,which should be considered the critical value. And the neonates should be screened by neonatal hemolytic disease. But the 1 ：64 titer determined by 2-Me neutrolization should not be considered critical value. The two methods of neutrolization should be considered different critical signs.